Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile-Reference-Cited by-同舟云学术

Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile

Published:2015-01 Issue:2 Volume:11 Page:233-249
ISSN:1479-6694
Container-title:Future Oncology
language:en
Short-container-title:Future Oncology

Author:

Kagohara Luciane Tsukamoto¹,Schussel Juliana L¹²,Subbannayya Tejaswini³,Sahasrabuddhe Nandini³,Lebron Cynthia¹,Brait Mariana¹,Maldonado Leonel¹,Valle Blanca L.¹,Pirini Francesca¹,Jahuira Martha¹,Lopez Jaime⁴,Letelier Pablo⁴⁵,Brebi-Mieville Priscilla¹⁴,Ili Carmen¹⁴,Pandey Akhilesh⁶⁷⁸⁹,Chatterjee Aditi¹³,Sidransky David¹,Guerrero-Preston Rafael¹⁸¹⁰

Affiliation:

1. Department of Otolaryngology–Head & Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA

2. Department of Oral Medicine, Post Graduation Program in Dentistry, Universidade Federal do Paraná, Curitiba, Brazil

3. Institute of Bioinformatics, Whitefield, Bangalore 560066, Karnataka, India

4. Universidad de La Frontera, Facultad de Medicina, Departamento de Anatomía Patológica, Laboratorio Patología Molecular, BIOREN-CEGIN, Temuco, Chile

5. Escuela Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile

6. McKusick–Nathans Institute of Genetic Medicine, Baltimore, MD 21205, USA

7. Department of Biological Chemistry, Baltimore, MD 21205, USA

8. Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

9. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

10. University of Puerto Rico School of Medicine, Department of Obstetrics & Gynecology, San Juan, Puerto Rico

Abstract

ABSTRACT Aim: The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile. Material & methods: DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR. Results: Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01) and ESR1 (p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation of APC, CDKN2A, ESR1, PGP9.5 and SSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%. Conclusion: Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.

Publisher

Future Medicine Ltd

Subject

Cancer Research,Oncology,General Medicine

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fon.14.165

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