A high-throughput multiplex genetic detection system for Helicobacter pylori identification, virulence and resistance analysis-Reference-Cited by-同舟云学术

A high-throughput multiplex genetic detection system for Helicobacter pylori identification, virulence and resistance analysis

Published:2016-10 Issue:10 Volume:11 Page:1261-1278
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Hu Binjie¹,Zhao Fuju¹,Wang Shiwen¹,Olszewski Michal A²,Bian Haipeng³,Wu Yong⁴,Kong Mimi⁴,Xu Lingli⁵,Miao Yingxin¹,Fang Yi¹,Yang Changqing³,Zhao Hu¹⁶⁷,Zhang Yanmei¹⁶⁷

Affiliation:

1. Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, No. 221 Yanan West Road, Shanghai, 200040, China

2. Division of Pulmonary & Critical Care Medicine, Department of Internal Medicine, University of Michigan Health System & Research Service, VA Ann Arbor Health Systems, Ann Arbor, MI, USA

3. Department of Gastroenterology, Tongji Hospital Affiliated to Shanghai Tongji University, Shanghai, China

4. Ningbo HEALTH Gene Technologies Co., Ltd., Ningbo, China

5. Shanghai ABSciex Analytical Instrument Trading Co., Ltd., Shanghai, China

6. Key Laboratory of Clinical Geriatric Medicine, Shanghai, China

7. Research Center on Aging & Medicine, Fudan University, Shanghai, China

Abstract

Aim: We established a high-throughput multiplex genetic detection system (HMGS) for identification of Helicobacter pylori with concomitant analysis of virulence and drug resistance. Materials & methods: Confirmed 132 H. pylori cultures from gastric biopsies were screened by 20-gene site-HMGS, sequencing and E-test. Results: HMGS was highly sensitive and specific for H. pylori identification. Concordance rate between HMGS and sequencing averaged 94.5% (virulence genes) and 97.3% (resistance genes). Observed resistance rates to four mainstream antibiotics were high, except for amoxicillin. Significant association between virulence genotype and risks for specific gastrointestinal diseases was found for five genes. Metronidazole resistance in peptic ulcer patients was significantly higher. Conclusion: HMGS is an effective method for H. pylori identification and analysis of virulence and drug resistance.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2016-0023

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