Ascribing a novel role for tmRNA of Escherichia coli in resistance to mitomycin C-Reference-Cited by-同舟云学术

Ascribing a novel role for tmRNA of Escherichia coli in resistance to mitomycin C

Published:2017-11 Issue:15 Volume:12 Page:1381-1395
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Shanmughapriya Vinod¹,Richard Stephen¹²,Nagarajan Tamilmaran¹,Munavar M Hussain¹

Affiliation:

1. Department of Molecular Biology, School of Biological Sciences, Centre for Advanced Studies in Functional & Organismal Genomics, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India

2. Faculty of Medicine, Technion – Israel Institute of Technology, Bat Galim, Haifa 31096, Israel

Abstract

Aim: The ssrA mutants were found to be more sensitive to mitomycin C (MMC) and our aim was to study this phenomenon in detail. Materials & methods: Strains were constructed by P1 transduction. pssrA+ plasmid was constructed by PCR-based cloning and transformation was done by CaCl2 method. Relative viability analyses were done to assess the extent of viability of strains in relevant conditions. Gram staining was used for microscopic analysis. Results: ssrA mutants become sensitive specifically to MMC, that too in a strain-specific manner. Precise tagging function of SsrA is necessary for conferring resistance to MMC. sulA::kan restored the viability of ssrA::cat mutants in a strain-specific manner. Conclusion: This study for the first time implicates SsrA in progression of efficient cell division and resistance to MMC.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2016-0148

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