Wirelessly powered and remotely controlled valve-array for highly multiplexed analytical assay automation on a centrifugal microfluidic platform

Author:

Delgado Sarai M. Torres,Kinahan David,Julius Lourdes Albina Nirupa,Mishra Rohit,Korvink Jan G.,Ducree Jens,Mager DarioORCID

Abstract

In this paper we present a wirelessly powered array of 128 centrifugo-pneumatic valves that can be thermally actuated on demand during spinning. The valves can either be triggered by a predefined protocol, wireless signal transmission via Bluetooth, or in response to a sensor monitoring parameters like temperature or homogeneity of the dispersion. Upon activation of a resistive heater, a low-melting membrane (ParafilmTM) is removed to vent an entrapped gas pocket, thus letting the incoming liquid wet an intermediate dissolvable film and thus open the valve. The proposed system allows up to 12 heaters to be activated in parallel with a response time below 3 seconds, potentially, resulting in 128 actuated valves in under 30 seconds. We demonstrate with three examples of common and standard procedures how the proposed technology could become a powerful tool for implementing diagnostic assays on Lab-on-a-Disc. First, we implement wireless actuation of 64 valves during rotation in a freely programmable sequence, or upon user input in real time.Then, we show a closed-loop centrifugal flow control sequence where the state of mixing between reagents evaluated from stroboscopically recorded images triggers the opening of valves. In our last experiment valving and closed-loop control are used to facilitate centrifugal processing of whole blood.

Publisher

Open Engineering Inc

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Automated Color-Making System for Dyeing Industry;International Journal of Advanced Research in Science, Communication and Technology;2023-04-11

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