Realization of malignant potential of human glioblastoma mitochondria in immunodeficient animals

Abstract

Purpose. Obtaining extracranial diffuse tumor foci of glioblastoma in Balb/c Nude mice by implantation of mitochondria isolated from human glioblastoma.Material and methods. Human glioblastoma mitochondria were isolated using differential centrifugation in a high speed refrigerated centrifuge. Mitochondrial samples were diluted with 0.9 % NaCl solution to a protein concentration of 3 mg of protein in 0.4 ml of saline. Balb/c Nude mice (N = 6) were intraperitoneally transplanted with freshly isolated glioblastoma mitochondria at the rate of 3 mg of protein per 1 animal in 0.4 ml of physiological solution. Male mice of Balb/c Nude line (N = 4), which were injected once intraperitoneally with 0.4 ml of physiological solution, served as control. All animals were euthanized by guillotine decapitation 17-21 days aſter parenteral implantation of mitochondria isolated from glioblastoma.Results. Specific changes in the spleen structure were revealed (N = 4): the formation of strands of cells resembling glioblastoma multi-forme was observed in multiple fields of vision. The initiation of diffuse growth of glioblastoma cells in the stromal part of the red pulp of the spleen was evidenced by the expression of GFAP and ki-67, which were also recorded in the tumor preparation of the patient with glioblastoma. Morphological examination of the mesentery and lymph nodes revealed multiple areas with atypical cells resembling glioblastoma multiforme, localized in fibrous septa and sinuses. Stained granules with the corresponding immunohistochemical profile, GFAP and ki-6, were clearly defined in the mesentery and lymph node of a Balb/c Nude mouse (N = 6) aſter transplantation of human glioblastoma mitochondria. An additional study of the immunophenotype of cells in the spleen, mesentery, and lymph nodes of Balb/c Nude mice by the generally accepted marker S100 leſt no doubt about the induction of malignant growth aſter transplantation of mitochondria isolated from human glioblastoma.Conclusions. Parenteral implantation of mitochondria isolated from human glioblastoma in male Balb/c Nude mice Might cause the growth and development of extracranial foci of glioblastoma in animals.