Author:
Darsini Ninik,Hamidah Berliana,Suyono Seso Sulijaya,Ashari Faisal Yusuf,Aswin R Haryanto,Yudiwati Rina
Abstract
The aim of this study was to analyze human sperm motility, viability, and morphology before and after cryopreservation. This true laboratory experimental study had pre and post randomized one group design. The study was conducted at the Embryology, Andrology, and Genetics Laboratory, Department of Medical Biology, Faculty of Medicine, Universitas Airlangga from August to November 2017. The eighteen samples of fresh semen were collected from male volunteers who agreed and signed the informed consent of the study. Samples were analyzed their motility, viability, and morphology before and after cryopreservation. Results of this study indicated differentiation between motility before and after cryopreservation. Cryopreservation process decreased progressive motility (42.22 + 9.46%; 17.83 + 6.24%; p< 0.0001) and increased the number of immotile spermatozoa (35.44 + 10.15%; 60.11 + 12.53%; p< 0.0001). Cryopreservation also decreased human sperm viability (73.78 + 8.91%; 40.83 + 12.89%; p< 0.0001) and morphology (10.94 + 4.96%; 7.39 + 3.90%; p< 0.0001). Cryopreservation of human spermatozoa caused the decreased of motility, viability, and morphology.
Reference10 articles.
1. Utility of antioxidants during assisted reproductive techniques: an evidence based review;Agarwal;Reproductive Biology and Endocrinology,2014
2. Cryopreservation of human spermatozoa decreases the number of motile normal spermatozoa, induces nuclear vacuolization and chromatin decondensation;Boitrelle;Journal of Andrology,2012
3. Cooper TG, Yeung CH (2010). Physiology of sperm maturation and fertilization. In: Nieschlag E, Behre HM, Nieschlag S (eds). Andrology, 3rd edition, Springer, 61-86
4. . Human sperm cryopreservation: Update on technique, effect on DNA integrity, and implications for ART;Di Santo;Advances in Urology,2012
5. . Cryopreservation of male gametes;Hossain;Infertility and Assisted Reproduction Cambridge Botroz and Juan Garcia,2008
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献