Հայկական և իրանական ցորենի սորտերի չորադիմացկանության համեմատումը ըստ՝ Wdhn13 և WCS726 դեհիդրին գեների էքսպրեսիայի և ջրի հարաբերական պարունակության տոկոսի

Abstract

In order to compare the level of drought resistance of fifteen Armenian and Iranian wheat varieties, the seeds were tested for 28 hours under drought stress and non-stress (controlled) conditions. Polyethylene Glycol 6000 (PEG6000) was used to preparing artificial drought conditions. Also we evaluate relative water content (RWC) and also expression level of Wdhn13 and WCS120 DHN genes by using reverse transcription PCR. According to the results, Navid, Sabala, Zare, and Voskehask varieties were highly resistant. The process of gene expression in these varieties began in the early hours, when the percentage of water content was still high. Reaching the peak in the 24th hour, it later began to decline slowly. In the second place were Azar 2, Sateni 22, and Akhtamar which could not maintain the high level of expression was decreased in the 24th hour. Sardari, Alvand, Pishgam, Nairi 68, and Armyanka 60 were in the third place, as the expression level decreased in those varieties already in the 18th hour. And Mihan, D92 և G31 were evaluated as sensitive varieties, because the expression of their genes began when the percentage of water content began to decrease. In these varieties, the decrease was noticeable already in the 12th hour. These two experiments together allow us to determine the resistance of varieties and by studying the mechanism of proteins for abiotic stresses, give us the ability to create resistant genotypes, improve the resistance of the genotypes, and obtain high yield characteristics.