Effectiveness of Giving Chitosan on Interleukin-6 and Mallondhyaldehide Levels in Wistar Rats with Chronic Periodontitis-Reference-Cited by-同舟云学术

Effectiveness of Giving Chitosan on Interleukin-6 and Mallondhyaldehide Levels in Wistar Rats with Chronic Periodontitis

Published:2023-03-20 Issue:1 Volume:7 Page:49-58
ISSN:2549-6395
Container-title:International Journal of Natural Science and Engineering
language:
Short-container-title:IJNSE

Author:

Dharmawati I Gusti Agung Ayu,Mastra Nyoman,Supariani Ni Nyoman Dewi,Ratih Gusti Ayu Made,Artini Ni Putu Rahayu

Abstract

The 6th highest diseases that occur in Indonesian society include dental caries and periodontitis caused by dental plaque which is a breeding ground for bacteria such as Streptococcus mutants and Phorphyromonas gingivalis. The purpose of this study was to determine the effectiveness of chitosan on levels of Interleukin-6 and Malondhyaldehyde (MDA), as an anti-inflammatory marker using the Elisa test. The research method was a true experimental randomized posttest only control group design, with 3 treatment groups namely control, 2% chitosan gel, and 100mg/200g/bb of chitosan orally in 15 Wistar rats with periodontitis induced by Phorphyromonas gingivalis bacteria. The results showed that the mean levels of Interleukin-6 and Mallondhyaldehyde were significantly different (p<0.05) between the control group, chitosan gel treatment, and oral chitosan administration Interleukin-6 levels were the highest in the chitosan gel (8.3091±1.25570), the control average (5.8523±0.51512), the lowest oral average (3.2563±1.45952). Similarly, Mallondhyaldehyde levels were highest in the gel group (2.8850±0.19353), control group (2.2284±0.00914), and oral group (1.2943±0.60280).). Based on these results, oral chitosan was the most effective in the treatment of periodontitis.

Publisher

Universitas Pendidikan Ganesha

Reference34 articles.

1. Abdel-Moneim, A., E.-, Shahawy, A., Yousef, A. I., Abd El-Twab, S. M., Elden, Z. E., & Taha, M. (2020). Novel polydatin-loaded chitosan nanoparticles for safe and efficient type 2 diabetes therapy: In silico, in vitro and in vivo approaches. International Journal of Biological Macromolecules, 154, 1496–1504. https://doi.org/10.1016/j.ijbiomac.2019.11.031.

2. Azuma, K., Osaki, T., Minami, S., & Okamoto, Y. (2015). Anticancer and anti-inflammatory properties of chitin and chitosan oligosaccharides. Journal of Functional Biomaterials, 6(1), 33–49. https://doi.org/10.3390/jfb6010033.

3. Campoccia, D., Montanaro, L., & Arciola, C. R. (2013). A review of the biomaterials technologies for infection-resistant surfaces. Biomaterials, 34(34), 8533–8554. https://doi.org/10.1016/j.biomaterials.2013.07.089.

4. Carvalho, C. V., Saraiva, L., Bauer, F. P. F., Kimura, R. Y., Souto, M. L. S., Bernardo, C. C., & Pustiglioni, F. E. (2018). Orthodontic treatment in patients with aggressive periodontitis. American Journal of Orthodontics and Dentofacial Orthopedics, 153(4), 550–557. https://doi.org/10.1016/j.ajodo.2017.08.018.

5. Chang, S. H., Lin, Y. Y., Wu, G. J., Huang, C. H., & Tsai, G. J. (2019). Effect of chitosan molecular weight on anti-inflammatory activity in the RAW 264.7 macrophage model. International Journal of Biological Macromolecules, 131, 167–175. https://doi.org/10.1016/j.ijbiomac.2019.02.066.