Detection and clinical manifestation of Epizootic Lymphangitis in horses in Zaria and Kontagora Emirates, Nigeria

Abstract

Introduction: A study was conducted to determine the clinical manifestation, isolation and identification of the aetiologic agent of epizootic lymphangitis in horses in Zaria and Kontagora Emirates in Nigeria. Methods: A total of seventeen (17) infected horses manifesting signs of epizootic lymphangitis were examined in Niger and Kaduna States of Nigeria. The pus samples were also examined using direct smears, Gram stained and examined under a light microscope. Infected lymphoid tissue samples were collected in 10% formal-saline from horses clinically manifesting infection for histopathological studies. Results: Clinical signs observed were painless swellings (nodules) and suppurative ulcers along lymphatic channels with some areas of partial healing and reoccurrence. Culture and direct smear revealed Gram positive double contour and oval shaped yeast-like organisms with a violet cytoplasm. Histopathological examination revealed Histoplasma farciminosum cells as oval bodies in macrophages. Significance: The study confirmed that Epizootic Lymphangitis is present in Nigeria.