Simultaneous UV Spectrophotometric Method for Estimation of Berberine and Withanolide in Polyherbal Immunity Booster Dosage Form

Abstract

The majority of commercial herbal preparations used to improve immunity lack standardization with regard to the active ingredients marker molecules. The objective of present study is the simultaneous estimation of berberine and withanolide in herbal immunity booster tablet using UV spectrophotometric method. The method was validated according to ICH guidelines. The results indicated that berberine and withanolide have maximal absorption wavelength i.e. λmax at 348 nm and 231 nm, respectively. Withanolide and berberine were found to behave linearly over the concentration range of 5–25μg/mL with R2 value of 0.9998 and 0.9999, respectively. Withanolide and berberine have shown % relative standard deviation (RSD) values of 0.07 and 0.09, respectively, in accuracy study. The intraday and interday precision was deemed adequate with a % RSD fewer than 2. Further, the limit of detection (LOD) and limit of quantization (LOQ) value for berberine and withanolide were found to be 0.12 mg/mL and 0.35mg/mL and 0.07, 0.20 mg/mL, respectively. In conclusion, the proposed method was validated in accordance with the International Conference on Harmonization (ICH) guidelines. The developed method can be employed to determine how much berberine and withanolide are present in pharmaceutical preparations.