Abstract
In this review article, we focus on in vitro antioxidant evaluation methods. Some emerging techniques (such as nanotechnology, sensors, electrochemistry, chemometry, and hyphenated methods) in antioxidant testing are broadly introduced and may be helpful for future prospects. These techniques are effective tools for the rapid isolation and screening of antioxidants (radical scavengers) present in complex mixtures such as plant extracts, foods, drugs and other low-colour compounds. Nanotechnology, sensors, electrochemistry, chemometry, and hyphenated techniques is simple to use, can reduce labour requirements and save time. Compounds with high antioxidant activity can shield biological systems from the potentially damaging effects of reactions involving reactive oxygen and nitrogen species. Information about the numerous techniques that can be used to support the positive effects of antioxidants. These cutting-edge innovations are extremely appealing because of affordability, efficiency, intuitiveness, simplified sample pre-treatment, great empathy, and phenomenal selectivity. The purpose of this review is imparting to significant data on modern technologies for detecting active antioxidant compounds. There are 19 in vitro techniques being utilized for antioxidant assessment. Numerous criteria can be used to classify antioxidants. The first characteristic (primary and secondary antioxidants) is determined by the algorithm. Understanding the antioxidant activity of various natural antioxidants and afterwards looking through them has gained popularity in recent years.
Publisher
Lloyd Institute of Management and Technology