Author:
Sharmaa Richa, ,Choudharyb Neeraj,Sharmac Gajanand, ,
Abstract
"A rise in mortality due to fungal infections in an immunocompromised population has been observed lately. Nowadays, due to increased fungal infections, the limitations encountered in their treatment like resistance, side-effects, and high toxicity, the rising prescription and overuse of conventional antifungals all stimulate a search for alternative natural drugs therefore we are in dire need of natural newer strategies that involve reliable agents for the treatment of fungal diseases such as essential oils are known for their anti-microbial properties and are multi-component. Materials & Methods: Soil samples (66 samples) were collected from different agricultural fields and animals habitat of Saharanpur (U.P.) Isolation of keratinophilic fungi was carried out by the hair-baiting technique. Extraction of Mentha piperita & Cinnamonum vernum essential oil was carried out by hydrodistillation method and the chemical composition of both extracted essential oils was determined by Gas ChromatographyMass Spectrophotometry. Antimycotic studies of essential oils were done by the standard disc diffusion method. Results & Discussion: In the present study, the antifungal potential of Mentha piperita & Cinnamonum vernum essential oils were evaluated against three human pathogenic fungi isolated from the soil of agricultural field and animals habitat of Saharanpur (U.P.) i.e Trichophyton mentagrophytes, T.tonsurans, and T.equinum. The chemical composition of Mentha piperita & Cinnamonum vernum EO was analyzed by GC-MS. Menthol (53.28%) was the major compound of the Mentha piperita EO followed by Menthyl acetate (15.1%) and Menthofuran (11.18%). Major constituents of Cinnamonum vernum EO were linalool (8%), (E)- cinnamaldehyde (7.2%), β-caryophyllene (7.4%), eucalyptol (6.4%), and eugenol (5.6%). Conclusion: Essential oils of Mentha piperita & Cinnamonum vernum have been found to have remarkable & excellent antifungal activity against these pathogenic fungi. Present findings conclude that natural products like plant-derived EOs instead of chemotherapy and the emergence of resistance to antifungal drugs can be regarded as an environmental safety mode of disease control against pathogens. "
Publisher
Asociatia Cadrelor Medicale din Spitalul Universitar de Urgenta Militar Central Dr. Carol Davila
Reference63 articles.
1. "1. Khanam S.J.P; Jain P.C. Isolation of keratin degrading fungi from soil of Damoh, India. Asian J Microbiol Biotech Environ Sci 2002, vol. 4, no 2, pp. 251-254.
2. 2. Mukesh S; Sharma M. Incidence of dermatophytes and other keratinophilic fungi in the schools and college playground soils of Jaipur, (India). African J Microbiol Res 2010, vol. 4, no 24, pp.2647- 2654.
3. 3. Marsella R; Mercantini R. Keratinophilic fungi isolated from soils of the Abruzzo National Park Italy. Mycopathol 1988, vol.94, no.2, pp.97-107.
4. 4. Dominik T; Majchrowicz I. A trial for isolating keratinolytic and keratinophilic fungi from the soils of the cemeteries and forests of Szczecin. Ekologia Polska - Seria A. 1964, vol.12, pp.79- 105.
5. 5. Ajello L. The dermatophytes, Microsporum gypseum as a saprophyte and parasite. J Investigat Dermatol. 1953, vol.21, no.3, pp.157-171. https://doi.org/10.1038/jid.1953.86