Author:
Astuti Rika Indri,Putera Saju Yohanes Bernadino,Aribah Dina,Wahyudi Aris Tri
Abstract
Marine bacteria associated with sponges can be used as a source of new enzymes, especially cellulase which are potentially used for various industries. The aim of this study was to screen and identify the cellulolytic bacteria associated with sponges. A total of 38 isolates have been successfully isolated from sponges Aaptos sp., Euryspongia sp., and Haliclona sp. by a spread plate method. Amongst 38 isolates, 14 (37%) showed cellulolytic activities in carboxy methyl cellulose (CMC) medium with a cellulolytic index ranging from 0.31 to 1.63. The three highest cellulolytic index was exhibited by bacterial isolates coded Y.5.10, Y.5.11, and Y.40.6. Isolates Y.5.10, Y.5.11, and Y.40.6 showed cellulolytic activity of 0.0053 U/mL; 0.0083 U/mL; and 0.0124 U/mL, respectively, as were tested by spectrophotometry. The highest specific enzymatic activity was showed by isolate Y.40.6 with a value of 0.3391 U/mg. Based on 16S rRNA gene, isolate Y.5.10, Y.5.11, and Y.40.6 were highly similar to (similarity ≥ 99%) Bacillus sp., B. subtilis, and B. cereus, respectively.
Keywords: cellulase, isolation, sponge-associated bacteria, 16S rRNA
Publisher
JIPI, Lembaga Penelitian dan Pengabdian kepada Masyarakat
Reference24 articles.
1. Ali N, Ting Z, Khan YH, Athar MA, Long M. 2014. Enzymatic hydrolysis of cellulosic biomass for the production of biofuels, a review. International Journal of Engineering and Technical Research. 2(12): 89–96.
2. Azadian F, Badoei-dalfard A, Namaki-Shoushtari A, Hassanshahian M. 2016. Purification and biochemical properties of a thermostable, haloalkaline cellulase from Bacillus licheniformis AMF-07 and its application for hydrolysis of different cellulosic substrates to bioethanol production. Miriam Budiardjo Resource Center. 5(3): 143–155.
3. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248–254. https://doi.org/10.1016/ 0003-2697(76)90527-3
4. dos Santos YQ, de Veras BO, de França AFJ, Gorlach-Lira K, Velasques J, Migliolo L, dos Santos EA. 2018. A new salt-tolerant thermostable cellulase from a marine Bacillus sp. strain. Journal of Microbiology Biotechnology. 28(7): 1078–1085. https://doi.org/10.4014/jmb.1802.02037
5. Fatokun EN, Nwodo UU, Okoh AI. 2016. Classical optimization of cellulase and xylanase production by a marine Streptomyces species. Applied Sciences 6(10): 286–299. https://doi.org/10.3390/ app6100286