DEVELOPMENT OF PRIMERS FOR IDENTIFICATION OF PARASITIC FUNGI OF THE GENUS RHYTISMA-Reference-Cited by-同舟云学术

DEVELOPMENT OF PRIMERS FOR IDENTIFICATION OF PARASITIC FUNGI OF THE GENUS RHYTISMA

Published:2024-06-28 Issue: Volume: Page:135-141
ISSN:
Container-title:Materials of the International Youth Scientific and Practical Conference "FORESTRY-BIOLOGICAL FOUNDATIONS OF SUSTAINABILITY OF NATURAL AND ARTIFICIAL PHYTOCOENOSES"
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Author:

Rzhevskiy Stanislav¹,Kondrat'eva Anna¹

Affiliation:

1. All-Russian research institute of forest genetics, selection and biotechnology

Abstract

This paper provides an overview of parasitic fungi of the genus Rhytisma found in Russia: Rhytisma acerinum (Pers.) Fr., Rh. punctatum (Pers.) Fr., the substrate for which is various types of maple, Rh. salicinum (Pers.) Fr., parasitizing willows and Rh. andromedae (Pers.) Fr. – on the Andromeda sp. Ascomycetes of this genus are the causative agents of black (tar) leaf spot, which damages the decorative qualities of plants, and at the same time, they can serve as indicators of the ecological situation. Types of rhytisma, for which maple leaves serve as a substrate, are widespread in Voronezh and its environs. The goal of this work was to develop, based on the sequences of these species available in the NCBI database, specific primers for their molecular genetic identification. Primer-Blast software was used to create them, and the specificity of the obtained sequences was checked in silico using the BLAST module on the NCBI website. Among the generated primer sequences, selection was made to match the optimal parameters. The use of molecular genetic analysis with these primers can be used for early diagnosis of pathogens, as well as in other cases of difficulties in the morphological determination of plant pathogen species.

Publisher

FSBE Institution of Higher Education Voronezh State University of Forestry and Technologies named after G.F. Morozov

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