Affiliation:
1. Voronezh state university
2. Federal State Budgetary Scientific Institution «All-Russian Research Institute of Sugar Beet and Sugar named after A.L. Mazlumova»
Abstract
The most important factor for the development and growth of a plant is light, which acts as the main source of energy. In addition, light regulates physiological and biochemical processes through photoreceptor systems. Studies have shown that microRNAs play a special regulatory role in the organization of cellular metabolism, regulating gene expression in various ways both at the transcriptional and post-transcriptional levels. There are a significant number of microRNAs that participate in the light-dependent mechanisms of regulation of target genes. miR165a microRNA is light-dependent, the amount of which varies depending on the state of the phytochrome system. The use of such a specific PEG 1500 coadjutor, which separates high-molecular and low-molecular RNAs during phenol-chloroform extraction, made it possible to obtain a high yield of microRNAs from the total cellular RNA. A probe and specific primers have been developed to quantify miR165a in corn leaves. It was found that the active form of phytochrome causes an increase in the concentration of the analyzed microRNA in the cells of corn leaves, which was shown using the real-time polymerase chain reaction method. A change in the light regime of light-loving plants, for example, corn, associated with a decrease in the proportion of red light in the light stream, will negatively affect the cellular regulation mediated by miR165a.
Publisher
FSBE Institution of Higher Education Voronezh State University of Forestry and Technologies named after G.F. Morozov