Effect of miRNA-99a along with TGF-?1(C/G +915) in female with secondary unexplained infertility

Abstract

Background: Women infertility is a big health problem worldwide and the genetic disorders considered underling causes of unexplained female infertility. Objective: to find out the role of miR-99a and TGF-?1 SNP (G/C +915) in development of secondary unexplained infertility in  women and prognostic of miR-99a. To achieve this goal, investigate the fold change of miR-99a and possibility to use them as biomarker. Also  identification of TGF-?1(G/C +915)SNP by ARMS- PCR. Material and methods: case control study have been conducted, blood samples had been collected from population (patients 50) and (control 50) living in the north area of Thi-Qar province The samples collected during the period from November 2021 to July 2022. The genomic RNA and DNA were extracted from serum and blood respectively for molecular assay. Results: the mean of miR-99a fold change in patients group 3.70 ±1.18 versus 0.84 ±0.34 in control group and p< 0.001. TGF-?1 SNP +915(GC) genotype CC was more frequent significantly in patients group than in control group (p < 0.001) and the odds ratio was 19.06, and no significant difference in frequency rate of genotype GC between study groups p = 0.106. Genotype GG was less frequent significantly in patients group than in control group p = 0.046 and the odds ratio was 0.22. Conclusion: we can get a diagnostic and prognostic as biomarker, using the miR-99a which show high sensitivity 100%,  specificity 98% and accuracy 100% in secondary unexplained infertility, also TGF? (+915 G/C) SNP as predisposing risk factor for developing of secondary unexplained infertility.