A new experimental model of cigarette smoke-induced emphysema in Wistar rats

Author:

Kozma Rodrigo de las Heras1,Alves Edson Marcelino2,Barbosa-de-Oliveira Valter Abraao1,Lopes Fernanda Degobbi Tenorio Quirino dos Santos2,Guardia Renan Cenize3,Buzo Henrique Vivi3,Faria Carolina Arruda de1,Yamashita Camila2,Cavazzana Junior Manzelio3,Frei Fernando2,Ribeiro-Paes Maria Jose de Oliveira3,Ribeiro-Paes Joao Tadeu2

Affiliation:

1. University of Sao Paulo, Brazil

2. Julio de Mesquita Filho Sao Paulo State University, Brazil

3. Faculdades Integradas Padre Albino, Brazil

Abstract

OBJECTIVE: To describe a new murine model of cigarette smoke-induced emphysema. METHODS: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). RESULTS: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. CONCLUSIONS: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.

Publisher

FapUNIFESP (SciELO)

Subject

Pulmonary and Respiratory Medicine

Reference29 articles.

1. Global Alliance against Chronic Respiratory Diseases (GARD). General Meeting Report; 2008 May 30-31; Istanbul, Turkey,2008

2. [homepage on the Internet,2011

3. Experimental basis and new insights for cell therapy in Chronic Obstructive Pulmonary Disease;de Faria CA;Stem Cell Rev,2012

4. Chronic Obstructive Pulmonary Disease - Current Concepts and Practice;Ribeiro-Paes JT,2012

5. Mechanisms of cigarette smoke-induced COPD: insights from animal models;Churg A;Am J Physiol Lung Cell Mol Physiol,2008

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3