Affiliation:
1. The University of Lahore, Pakistan
2. The University of Lahore, Pakistan; Kinnaird College For Women, Pakistan
3. University of the Punjab, Pakistan
Abstract
Abstract Bone marrow-derived mesenchymal stromal cells (BMSCs) have been used for treating inflammatory disorders. Due to the large size of BMSCs compared to nanoparticles, BMSCs cannot be loaded into the nanoparticles. It is hypothesized that BMSCs lysate loading into the nanocarriers will effectively deliver cellular contents and regulatory elements of BMSCs at the injury site. This study aimed to investigate nanostructured lipid carriers (NLC) loading with BMSCs lysate through basic characterization and morphological analysis. Moreover, this study was mainly designed to investigate the role of NLC loaded BMSCs lysate in reducing inflammation via in-vitro and in-vivoassays. The in-vitro study involves cell viability assays, p53, annexin V and VEGF expression through ELISA and immunocytochemistry, real-time BAX, caspase-3, IL-6, IL-8, TOP2A, PCNA, and Ki-67 gene expression analysis. Additionally, to evaluate in-vivo anti-inflammatory activity, the carrageenan-induced rat paw oedema model was used. In-vitro results showed that NLC loaded BMSCs lysate increased cell viability, decreased apoptosis and pro-inflammatory genes expression and up-regulated angiogenesis and proliferation in H2O2 pre-stimulated cells. Findings of the in-vivo assay also indicated a reduction in rat's paw oedema volume in NLC-loaded BMSCs lysate, and downregulation of BAX, Caspase-3, IL-6, and IL-8 was observed. Enhanced expressions of TOP2A, PCNA, and Ki-67 were obtained. Concluding the results of this study, NLC-loaded BMSCs lysate could reduce inflammation and possibly regenerate damaged tissue mainly via increasing cell viability, angiogenesis and proliferation, and reducing apoptosis and pro-inflammatory cytokines.
Subject
General Agricultural and Biological Sciences
Reference40 articles.
1. Comparison of clinical and biochemical variables between patients on twice a week dialysis regime having different residual renal function: a jack in the box;AFZAL A.;Biological and Clinical Sciences Research Journal,2022
2. A review of inflammatory mechanism in airway diseases;AGHASAFARI P.;Inflammation Research,2019
3. N-Acetyl cysteine protects diabetic mouse derived mesenchymal stem cells from hydrogen-peroxide-induced injury: A novel hypothesis for autologous stem cell transplantation;ALI F.;Journal of the Chinese Medical Association,2016
4. Compatibility analysis of bergapten with different pharmaceutical excipients used in nanostructured lipid carriers;ARSHAD S.;Pakistan Journal of Pharmaceutical Sciences,2019
5. Cassia fistula seed extract enhances apoptosis in HeLa cell line via activating annexin V and p53;AWAN S.J.;Science Letters,2017