Affiliation:
1. Instituto Federal de Educação, Brasil
2. Universidade de São Paulo, Brasil
3. Universidade de Franca, Brasil
4. Universidade Federal de Uberlândia, Brasil
Abstract
Abstract Xylella fastidiosa is a plant-pathogenic bacterium that lives inside host xylem vessels, where it forms biofilm which is believed to be responsible for disrupting the passage of water and nutrients. Pectobacterium carotovorum is a Gram-negative plant-specific bacterium that causes not only soft rot in various plant hosts, but also blackleg in potato by plant cell wall degradation. Chagas disease, which is caused by Trypanosoma cruzi, has been commonly treated with nifurtimox and benzonidazole, two drugs that cause several side effects. As a result, the use of natural products for treating bacterial and neglected diseases has increased in recent years and plants have become a promising alternative to developing new medicines. Therefore, this study aimed to determine, for the first time, the chemical composition of essential oil from Psidium guajava flowers (PG-EO) and to evaluate its in vitro anti-Xylella fastidiosa, anti-Pectobacterium carotovorum, anti-Trypanosoma cruzi and cytotoxic activities. PG-EO was obtained by hydrodistillation in a Clevenger apparatus while its chemical composition was determined by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Major compounds identified in PG-EO were α-cadinol (37.8%), β-caryophyllene (12.2%), nerolidol (9.1%), α-selinene (8.8%), β-selinene (7.4%) and caryophyllene oxide (7.2%). Results showed that the PG-EO had strong trypanocidal activity against the trypomastigote forms of Trypanosoma cruzi (IC50 = 14.6 μg/mL), promising antibacterial activity against X. fastidiosa (MIC = 12.5 μg/mL) and P. carotovorum (MIC = 62.5 μg/mL), and moderate cytotoxicity against LLCMK2 adherent epithelial cells in the concentration range (CC50 = 250.5 μg/mL). In short, the PG-EO can be considered a new source of bioactive compounds for the development of pesticides and trypanocide drugs.
Subject
General Agricultural and Biological Sciences
Reference53 articles.
1. In identification of essential oil components by gas chromatography/quadrupole mass spectroscopy;ADAMS R.P.,2007
2. Antimicrobial, antioxidante, and cytotoxic activities of Ocimum forskolei and Teucrium yemense (Lamiaceae) essential oils;ALI N.A.A.;Medicines (Basel, Switzerland),2017
3. Biological activity of the essential oils from Cinnamodendron dinisii and Siparuna guianensis.;ANDRADE M.A.;Brazilian Journal of Microbiology,2015
4. Chemical composition and evaluation of antileishmanial and cytotoxic activities of the essential oil from leaves of Cryptocarya aschersoniana Mez. (Lauraceae Juss.);ANDRADE P.M.;Anais da Academia Brasileira de Ciências,2018
5. Potential roles of essential oils on controlling plant pathogenic bacteria Xanthomonas species: a review;BAJPAI V.K.;The Plant Pathology Journal,2011
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