Affiliation:
1. PMAS-Arid Agriculture University, Pakistan
2. MNS-University of Agriculture, Pakistan
3. PMAS-Arid Agriculture University, Pakistan; MNS-University of Agriculture, Pakistan
Abstract
Abstract Cucumber mosaic virus (CMV) is a tremendous threat to vegetables across the globe, including in Pakistan. The present work was conducted to investigate the genetic variability of CMV isolates infecting pea and spinach vegetables in the Pothwar region of Pakistan. Serological-based surveys during 2016-2017 revealed 31.70% overall CMV disease incidence from pea and spinach crops. Triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) revealed that all the positive isolates belong to CMV subgroup II. Two selected cDNA from ELISA-positive samples representing each pea and spinach crops were PCR-amplified (ca.1100 bp) and sequenced corresponding to the CMV CP gene which shared 93.7% nucleotide identity with each other. Both the sequences of CMV pea (AAHAP) and spinach (AARS) isolates from Pakistan were submitted to GenBank as accession nos. MH119071 and MH119073, respectively. BLAST analysis revealed 93.4% sequence identity of AAHAP isolate with SpK (KC763473) from Iran while AARS isolate shared maximum identity (94.5%) with the strain 241 (AJ585519) from Australia and clustered with some reference isolates of CMV subgroup II from UK (Z12818) and USA (AF127976) in a Neighbour-joining phylogenetic reconstruction. A total of 59 polymorphic (segregating) sites (S) with nucleotide diversity (π) of 0.06218 was evident while no INDEL event was observed in Pakistani isolates. The evolutionary distance of Pakistani CMV isolates was recorded as 0.0657 with each other and 0.0574-0.2964 with other CMV isolates reported elsewhere in the world. A frequent gene flow (Fst = 0.30478 <0.33) was observed between Pakistani and earlier reported CMV isolates. In genetic differentiation analysis, the value of three permutation-based statistical tests viz; Z (84.3011), Snn (0.82456), and Ks* (4.04042) were non-significant. The statistical analysis revealed the values 2.02535, 0.01468, and 0.71862 of Tajima's D, Fu, & Li’s F* and D* respectively, demonstrating that the CMV population is under balancing selection.
Subject
General Agricultural and Biological Sciences
Reference61 articles.
1. First report of a Cucumber mosaic virus (CMV) subgroup II isolate infecting pea in Pakistan;AHSAN M.;Journal of Plant Pathology,2018
2. Current status and genetic variability of Cucumber mosaic cucumovirus (CMV) isolates infecting major cucurbits and solanaceous vegetables in Pothwar region of Pakistan;AHSAN M.;Pakistan Journal of Agricultural Sciences,2020
3. Occurrence of Cucumber mosaic virus subgroup IA in tomato in Pakistan;AKHTAR K.P.;Journal of Plant Diseases and Protection,2008
4. Seed transmission of Cucumber mosaic virus in pepper;ALI A.;Journal of Virological Methods,2010
5. Screening for resistance to Cucumber mosaic cucumovirus in chilli pepper;ASHFAQ M.;Journal of Animal and Plant Sciences,2014
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