Affiliation:
1. University of Karachi, Pakistan; Ziauddin University, Pakistan
2. University of Karachi, Pakistan
3. Jinnah Sindh Medical University, Pakistan
4. Ziauddin University, Pakistan
Abstract
Abstract Environmental pollutants and lifestyle severely threaten human and animal health, leading to disturbances of various functions, including infertility. So, exploring a safe treatment that could effectively reverse infertility remains a challenge. The current study was intended to explore the fertility-enhancing effect of Juglans Regia oil in two successive generations of rats; F0 and F1. J. Regia oil was initially tested for in vitro antioxidant assay via ROS and DPPH, followed by in vivo toxicity testing. In the fertility assessment, eighteen pairs of male and female rats (n=36, 1:1, F0 generation) were divided into three groups and dosed with 1 mL/kg and 2 mL/kg daily of J. Regia oil and saline, respectively, up to pre-cohabitation, cohabitation, gestation and lactation periods. The reproductive performance, including body weight, live birth index, fertility index, and litter size, was assessed. Hormonal and antioxidant markers of F1 generations were assessed with the histopathological evaluation of male and female organs. The oil of J. Regia showed great antioxidant potential (P < 0.05) in DPPH (1,1-diphenyl-2-picrylhydrazyl) and ROS (Reactive Oxygen Species) methods (P<0.05). The continued exposure of the F0 and F1 generations to J. Regia oil did not affect body weight, fertility index, litter size, and survival index. We have found pronounced fertility outcomes in both genders of F0 and F1 generations with J. Regia 2 mL/kg/day in comparison to the control. Results showed that J. Regia significantly increased (P < 0.05) luteinizing hormone (LH), plasma testosterone, follicular stimulating hormone (FSH), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities in both generations. Histology of both generations reveals improved spermatogenesis and folliculogenesis with enhanced architecture. Altogether, the present results suggest that J. Regia improved fertility in both male and female rats by improving hormonal activities and oxidative stress.
Subject
General Agricultural and Biological Sciences
Reference66 articles.
1. The use of plant extracts to control tilapia reproduction: current status and future perspectives;ABAHO I.;Journal of the World Aquaculture Society,2022
2. Phytochemical screening and antimalarial activity of some plants traditionally used in Indonesia;ABDILLAH S.;Asian Pacific Journal of Tropical Disease,2015
3. Nitrite-induced testicular toxicity in rats: therapeutic potential of walnut oil;ADELAKUN S.A.;JBRA Assisted Reproduction,2019
4. Nitrite-induced testicular toxicity in rats: therapeutic potential of walnut oil;ADELAKUN S.A.;JBRA Assisted Reproduction,2019
5. The effects of Ficus carica on male and female reproductive capabilities in rats;AIN N.U.;Evidence-Based Complementary and Alternative Medicine,2022