Low-P tolerance mechanisms and differential gene expression in contrasting wheat genotypes

Author:

Espindula Laize Fraga1,Minella Euclydes2,Delatorre Carla Andréa1

Affiliation:

1. Universidade Federal do Rio Grande do Sul, Brazil

2. Embrapa Trigo, Brazil

Abstract

The objectives of this study were to determine low-P tolerance mechanisms in contrasting wheat genotypes and to evaluate the association of these mechanisms to differential gene expression. Wheat seedlings of cultivars Toropi (tolerant to low-P availability) and Anahuac (sensitive) were evaluated. Seedlings were hydroponically grown in the absence or presence of P (1.0 mmol L-1) during three different time periods: 24, 120 and 240 hours. Free phosphate (Pi) and total P contents were measured in shoots and roots. The experiment's design was in randomized blocks with three replicates, each formed by ten plants. The relative expression of genes encoding the malate transporter TaALMT1 and the transcription factor PTF1 was evaluated. Phosphorus starvation beyond ten days increased the expression of TaALMT1 only in 'Toropi'. PTF1's expression was early induced in both genotypes under P starvation, but remained significant after ten days only in 'Toropi'. Shoot Pi concentration in 'Toropi' was independent from P availability; under starvation, 'Toropi' favored the maintenance of shoot Pi concentration. The low-P tolerance of Toropi cultivar at initial growth stages is mainly due to its ability to maintain constant the Pi shoot level.

Publisher

FapUNIFESP (SciELO)

Subject

Agronomy and Crop Science,Animal Science and Zoology

Reference32 articles.

1. Phosphate sensing in higher plants;ABEL S.;Physiologia Plantarum,2002

2. Absorção, translocação e utilização de fósforo por variedades de trigo submetidas à toxidez de alumínio;ABICHEQUER A.D.;Revista Brasileira de Ciência do Solo,2003

3. Assay of inorganic phosphate, total phosphate and phosphatases;AMES B.N.;Methods in Enzymology,1966

4. A world dataset of derived soil properties by FAO-UNESCO soil unit for global modeling;BATJES N.H.;Soil Use and Management,1997

5. Resposta diferencial de genótipos de trigo em relação a fósforo no solo;BEN J.R.;Pesquisa Agropecuária Brasileira,1998

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3