Affiliation:
1. Embrapa Arroz e Feijão
2. Embrapa Recursos Genéticos e Biotecnologia
Abstract
The objective of the present work was to determine the inheritance and stability of transgenes of a transgenic bean line expressing the genes rep-trap-ren from Bean golden mosaic virus and the bar gene. Crosses were done between the transgenic line and four commercial bean cultivars, followed by four backcrosses to the commercial cultivars. Progenies from each cross were evaluated for the presence of the transgenes by brushing the leaves with glufosinate ammonium and by polymerase chain reaction using specific oligonucleotides. Advanced generations were rub-inoculated with an isolate of Bean common mosaic necrosis virus (BCMNV). The transgenes were inherited consistently in a Mendelian pattern in the four crosses studied. The analyzed lines recovered close to 80% of the characteristics of the recurrent parent, as determined by the random amplified DNA markers used, besides maintaining important traits such as resistance to BCMNV. The presence of the transgene did not cause any detectable undesirable effect in the evaluated progenies.
Subject
Agronomy and Crop Science,Animal Science and Zoology
Reference27 articles.
1. Genetic transformation of peanut (Arachis hypogea L;ANURADHA T.S.;) using cotyledonary node as explant and a promoterless gus:nptII fusion gene based vector. Journal of Biosciences,2006
2. Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L;ARAGÃO F.J.L.;) co-transformed via particle bombardment. Theoretical and Applied Genetics,1996
3. Transgênico resistente a Geminivirus;ARAGÃO F.J.L.;Biotecnologia,2001
4. Resistance to Bean Golden Mosaic Virus in bean genotypes;BIANCHINI A.;Plant Disease,1999
5. Inheritance and segregation of virus and herbicide resistance transgenes in sugarcane;BUTTERFIELD M.K.;Theoretical and Applied Genetics,2002
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献