Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows-Reference-Cited by-同舟云学术

Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows

Published:2016-05 Issue:5 Volume:51 Page:676-679
ISSN:0100-204X
Container-title:Pesquisa Agropecuária Brasileira
language:
Short-container-title:Pesq. agropec. bras.

Author:

Oliveira Andre Penido¹,Heinemann Marcos Bryan²,Cortez Adriana³,Nascimento Paula Maria Pires do²,Leite Romulo Cerqueira²,Viana João Henrique Moreira⁴

Affiliation:

1. Empresa de Pesquisa Agropecuária de Minas Gerais, Brazil

2. Universidade Federal de Minas Gerais, Brazil

3. Universidade Santo Amaro, Brazil

4. Embrapa Gado de Leite, Brazil

Abstract

Abstract: The objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV-1) in follicular fluid and in cumulus-oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid. Viral DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV-1 by COC or follicular fluid.

Publisher

FapUNIFESP (SciELO)

Subject

Agronomy and Crop Science,Animal Science and Zoology

Link

http://www.scielo.br/pdf/pab/v51n5/1678-3921-pab-51-05-00676.pdf

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2. Impairment on nuclear maturation rate in oocytes from cows naturally infected by bovine herpesvirus 1 (BoHV-1);Pesquisa Veterinária Brasileira;2018-12