Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter-Reference-Cited by-同舟云学术

Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

Published:2013-07 Issue:7 Volume:48 Page:741-747
ISSN:0100-204X
Container-title:Pesquisa Agropecuária Brasileira
language:
Short-container-title:Pesq. agropec. bras.

Author:

Attílio Lísia Borges¹,Mourão Filho Francisco de Assis Alves¹,Harakava Ricardo²,Silva Tatiane Loureiro da¹,Miyata Luzia Yuriko¹,Stipp Liliane Cristina Libório¹,Mendes Beatriz Madalena Januzzi³

Affiliation:

1. Escola Superior de Agricultura Luiz de Queiroz, Brazil

2. Instituto Biológico, Brazil

3. Centro de Energia Nuclear na Agricultura, Brazil

Abstract

The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co‑cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real‑time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.

Publisher

FapUNIFESP (SciELO)

Subject

Agronomy and Crop Science,Animal Science and Zoology

Link

http://www.scielo.br/pdf/pab/v48n7/06.pdf

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