Fungitoxic activity avaliation of the hexane and methanol extracts of copaiba plant leavesCopaifera langsdorffi Desfon

Abstract

The fungitoxicity of hexane and methanol extracts of copaiba plants were evaluated by biological tests against the phytopathogens Colletotrichum gloeosporioides and Bipolaris sorokiniana. These extracts were chosen because they presented the highest yields in the extraction at room temperature. The biological material was obtained from the Department of Phytopathology of the UFLA, transplanted in BDA (C. gloeosporioides) and PCA (B. sorokiniana) culture media and incubated in a germination chamber with control of light and temperature. Five treatments (0/100/200/400/800 ppm) were used with three repetitions of each, and four mutually perpendicular radii, were measured in centimeters from the mycelial center. The formula for the Mycelial Growth Index (MGI) was applied to the values obtained for statistical analyses. A 32% reduction in the growth of B. sorokiniana was observed in the presence of the methanol extract, while the hexane extract was effective against both of the phytopathogens, resulting in a 49% reduction in the growth of B. sorokiniana and an 18% reduction in the growth of C. gloeosporioides.