Comparison of predicted binders in Rhipicephalus (Boophilus) microplus intestine protein variants BM86 Campo Grande strain, BM86 and BM95

Author:

Andreotti Renato1,Pedroso Marisela S.2,Caetano Alexandre R.3,Martins Natália F.3

Affiliation:

1. Embrapa Gado de Corte, Brazil

2. Centro de Ingeniería Genética y Biotecnologia, Cuba

3. Embrapa Recursos Genéticos e Biotecnologia, Brazil

Abstract

This paper reports the sequence analysis of Bm86 Campo Grande strain comparing it with Bm86 and Bm95 antigens from the preparations TickGardPLUS and GavacTM, respectively. The PCR product was cloned into pMOSBlue and sequenced. The secondary structure prediction tool PSIPRED was used to calculate alpha helices and beta strand contents of the predicted polypeptide. The hydrophobicity profile was calculated using the algorithms from the Hopp and Woods method, in addition to identification of potential MHC class-I binding regions in the antigens. Pair-wise alignment revealed that the similarity between Bm86 Campo Grande strain and Bm86 is 0.2% higher than that between Bm86 Campo Grande strain and Bm95 antigens. The identities were 96.5% and 96.3% respectively. Major suggestive differences in hydrophobicity were predicted among the sequences in two specific regions.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Parasitology

Reference27 articles.

1. BmTI antigens induce a bovine protective immune response against Boophilus microplus tick.;ANDREOTTI R.;International Immunopharmacology,2002

2. Performance of two Bm86 antigen vaccine formulation against tick using crossbreed bovines in stall test.;ANDREOTTI R.;Revista Brasileira de Parasitologia Veterinária,2006

3. Generation and maintenance of diversity in the cattle MHC class I region.;BIRCH J.;Immunogenetics,2006

4. Reproductive and safety assessment of the vaccination with GavacTM against the cattle tick (Boophilus microplus).;BOUÉ O.;Theriogenology,1999

5. Immune control of Babesia bovis infection.;BROWN W.C.;Veterinary Parasitology,2006

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3