Evaluation of techniques for diagnosis of Trypanosoma vivax infections in naturally infected cattle in the Zona da Mata Mineira

Author:

Alcindo Jefferson Filgueira1ORCID,Vieira Maria Clara Guimarães1,Rocha Thamiris Vilela Pereira1,Cardinot Cinthya Brillante1,Deschk Maurício1,Amaral Gláucia Guimarães1,Araujo Rafael Ferreira de1,Franciscato Carina1,Castilho Neto Kayo José Garcia de Almeida2,Machado Rosângela Zacarias2,André Marcos Rogério2

Affiliation:

1. Universidade Federal de Juiz de Fora, Brasil

2. Universidade Estadual Paulista, Brasil

Abstract

Abstract This study aimed to evaluate diagnostic techniques for trypanosomiasis, caused by Trypanosoma vivax, in naturally infected cattle in Minas Gerais, Zona da Mata. The deaths of six lactating cows with similar clinical conditions—characterized by hyporexia, hypogalactia, and recumbency—had been reported from one property. Initially, two animals were examined and diagnosed with trypanosomiasis through identification of the protozoan in a blood smear. After the initial diagnosis, all lactating cows (n=37) on the property were examined, and blood samples were collected for tests including whole blood smear, buffy coat smear, Woo's technique, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). Woo's test, buffy coat smears, and whole blood smears indicated that 4/37 (10.81%) animals were positive for trypanosomiasis, whereas ELISA and PCR indicated that 33/37 (89.19%) and 27/37 (72.97%) animals, respectively, were positive. The agreement obtained between parasitological techniques was classified as high, while between ELISA and PCR, no agreement. In conclusion, parasitological techniques have a low capacity to identify infected animals in the chronic stage of T. vivax infection. Therefore, techniques such as PCR and/or ELISA should be used to minimize the occurrence of false negatives.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Parasitology

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