Affiliation:
1. UFC, Brazil
2. Surgical Pathology and Cytology Laboratory, Brazil
3. UFC, Brazi
Abstract
PURPOSE: To evaluate the effects of pre-conditioning with L-alanyl- glutamine (L-Ala-Gln) in rats subjected to total hepatic ischemia. METHODS: Thirty Wistar rats, average weight 300g, were randomly assigned to 3 groups (n=10): G-1 - Saline, G-2- L-Ala-Gln, G-3-control (Sham). G-1 and G-3 groups were treated with saline 2.0 ml or L-Ala-Gln (0.75mg/Kg) intraperitoneally (ip) respectively, 2 hours before laparotomy. Anesthetized rats were subjected to laparotomy and total hepatic ischemia (30 minutes) induced by by clamping of portal triad. Control group underwent peritoneal puncture, two hours before the sham operation (laparotomy only). At the end of ischemia (G1 and G2), the liver was reperfused for 60 minutes. Following reperfusion blood samples were collected for evaluation of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels. Liver (medium lobe) was removed for immunohistochemistry study with antibody for Caspase-3. RESULTS: It was found a significant decrease (p<0.05) of ALT levels (270.6 +40.8 vs 83.3 +5.5 - p <0.05), LDH (2079.0 +262.4 vs. 206.6 +16.2 - p <0.05) and Caspase-3 expression (6.72 +1.35 vs. 2.19 +1.14, p <0.05) in rats subjected to I / R, comparing the group treated with L-Ala -Gln with G-2. Also, the ALT level was significantly lower (P<0.05) in G-1 and G-2 groups than in G-3 (control group). CONCLUSION: L-Ala-Gln preconditioning in rats submitted to hepatic I/R significantly reduces ALT, LDH and Caspase-3 expression, suggesting hepatic protection.
Reference24 articles.
1. As bases experimentais da lesão por isquemia e reperfusão do fígado;Miranda LE;Acta Cir Bras.,2004
2. Aspectos básicos da lesão de isquemia e reperfusão e do pré-condicionamento isquêmico;Silva OC;Acta Cir Bras.,2002
3. Notes of the arrest of hepatic hemorrhage due to trauma;Pringle JH;Ann Surg.,1908
4. Ranitidine reduces ischemia/reperfusion-induced liver injury in rats by inhibiting neutrophil activation;Okajima K;J Pharmacol Exp Ther.,2002
5. Effects of alpha tocopherol and verapamil on liver and small bowel following mesenteric ischemia-reperfusion;Yagmurdur MC;Turk J Gastroenterol.,2002
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