ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS

Author:

Silva Isamara Ferreira da1ORCID,Guimarães Amanda Leite1ORCID,Amorim Vinícius Santana1ORCID,Silva Telma Maria Guedes da2ORCID,Peixoto Rodolfo de Moraes3ORCID,Nunes Xirley Pereira1ORCID,Silva Tânia Maria Sarmento2ORCID,Costa Mateus Matiuzzi da1ORCID

Affiliation:

1. Universidade do Vale do São Francisco, Brazil

2. Universidade Federal Rural de Pernambuco, Brazil

3. Instituto Federal do Sertão Pernambucano, Brazil

Abstract

Abstract Considering the therapeutic potential of medicinal plants as alternatives to antibiotic therapy, the research aims to evaluate the antimicrobial activity of Commiphora leptophloeos against isolates of Staphylococcus spp. from the milk of ruminants with subclinical mastitis. For this, the crude ethanolic extract from the bark and leaves of Commiphora leptophloeos was prepared, with these being chemically characterized by HPLC-DAD-MS and by MALDI-TOF. The extracts were then evaluated as to their antimicrobial effects against 60 isolates of Staphylococcus spp. through the broth microdilution technique to determine the minimum bactericidal concentration. In addition, the extracts were evaluated as to their ability to interfere with biofilm formation and with the already established biofilm. Although all tested extracts showed antimicrobial action, lower MBC values were recorded for the bark extract in the concentration 781.2 µg/mL (25/60). The extracts of the bark and leaves were able to interfere with the initial stages of biofilm formation, but there was no interference of the extract on the established biofilm. There was a high sensitivity of Staphylococcus spp. isolates from subclinical mastitis cases in ruminants when subjected to the extracts from bark and leaves of Commiphora leptophloeos, as well as regarding the ability of extracts to interfere in biofilm formation, indicating their potential in the use for ruminant mastitis therapy.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Animal Science and Zoology

Reference51 articles.

1. Expression patterns of β-defensin and cathelicidin genes in parenchyma of bovine mammary gland infected with coagulase-positive or coagulase-negative Staphylococci;Kościuczuk EM;BMC Veterinary Research,2014

2. Altered Leukocyte Responsiveness in Dairy Cows with Naturally Occurring Chronic Staphylococcus aureus Mastitis;Nagahata H;Journal of Veterinary Medical Science,2011

3. Prevalence, somatic cell count and etiology of bovine mastitis in Cuban herds from Mayabeque province using hand and machine milking;Ruiz AK;Revista Salud. Animal,2014

4. Epidemiological and bacteriological survey of buffalo mastitis in Nepal;Dhakal IP;Journal Veterinary Medical Science,2007

5. Incidences of and genetic parameters for mastitis, claw disorders, and common health traits recorded in dairy cattle contract herds;Gernand E;Journal of Dairy Science,2012

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3