ELISA of amyloid A in paired bronchoalveolar lavage fluid and serum samples of healthy horses

Author:

Filippo Paula Alessandra Di1ORCID,Ribeiro Luiza M.F.1,Meireles Marcos A.D.1,Lannes Saulo T.1,Mello Luciana. M.1,Gobbi Francielli P.1,Toledo Luiz F.A.2,Lessa Daniel A.B.2

Affiliation:

1. Universidade Estadual do Norte Fluminense, Brazil

2. Universidade Federal Fluminense, Brazil

Abstract

ABSTRACT: Pulmonary disorders are common in horses, and treatment efficiency depends on an adequate diagnosis. Amyloid A is the most sensitive indicator of pathology in horses. The objective of this study was to establish the concentration of amyloid A of bronchoalveolar lavage fluid (BALF) in healthy horses. Health condition of horses was considered normal based on physical examination, complete blood count, biochemical parameters, and BALF cytology. Blood and BALF were collected from thirty adult female horses. Amyloid A concentrations in serum and BALF were measured using commercial ELISA tests. Amyloid A was detected in serum (mean ± SD = 3.71±2.51) and BALF (mean ± SD = 0.000745±0.000785) of all horses. In conclusion, SAA can also be measured in bronchoalveolar fluid, affording early detection of respiratory infections or inflammatory conditions.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

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