Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil-Reference-Cited by-同舟云学术

Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil

Published:2018-11 Issue:11 Volume:38 Page:2037-2043
ISSN:1678-5150
Container-title:Pesquisa Veterinária Brasileira
language:
Short-container-title:Pesq. Vet. Bras.

Author:

Bier Daniele¹,Kich Jalusa D.²,Duarte Sabrina C.²,Silva Márcio R.³,Valsoni Luiza M.¹,Ramos Carlos A.N.⁴,Rodrigues Dália P.⁵,Araújo Flábio R.⁶

Affiliation:

1. Universidade Católica Dom Bosco, Brazil

2. Embrapa Suínos e Aves, Brazil

3. Embrapa Gado de Leite, Brazil

4. Universidade Federal de Mato Grosso do Sul, Brazil

5. Instituto Oswaldo Cruz, Brazil

6. Embrapa Gado de Corte, Brazil

Abstract

ABSTRACT: The aim of the present study was to investigate the presence of Salmonella spp. in samples collected from beef meat at three points of the slaughter line (after skinning, washing and cooling) at three slaughterhouses in Brazil that export meat. Detection was based on ISO 6579:2002 and confirmed by PCR and qPCR. The isolates were typified using slide agglutination tests and PFGE. The antibiotic sensitivity profile was determined using the disk diffusion method. Contamination was detected in only one slaughterhouse. The overall frequency of contamination by Salmonella spp. was 6.7% of carcasses (6/90) and 2.6% of carcass surface samples (7/270). All isolates were confirmed by PCR and qPCR. The serological analysis and the PFGE showed a single profile: Typhimurium. The strains demonstrated 100% susceptibility to ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin and tetracycline. Positive carcasses after cooling pose a direct risk to consumers, since the meat is considered ready to be marketed after this process.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

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