Affiliation:
1. Universidade Federal do Vale do São Francisco, Brasil
2. Universidade Federal do Vale do São Francisco, Brasil; Universidade Federal de Minas Gerais, Brasil
3. Universidade Estadual do Maranhão, Brasil
4. Fundação Oswaldo Cruz, Brasil
5. Netherlands Hanseniasis Relief, Brasil
Abstract
Abstract The aim of this study was to investigate the level of exposure to Leishmania infection in stray dogs in an area of intense visceral leishmaniasis transmission in the state of Pernambuco, Brazil. Blood samples from 178 dogs were analyzed using serological and molecular assays: rapid immunochromatographic test (ICT), enzyme-linked immunosorbent assay (ELISA), immunofluorescence antibody test (IFAT), and conventional and quantitative polymerase chain reaction (cPCR and qPCR). Positivity values obtained with serological tests were 71.4% (127/178), 70.2% (125/178), and 50.6% (90/178) using ICT, ELISA, and IFAT, respectively, with 38.8% (69/178) of the dogs were simultaneously positive for all three tests. The positivity values obtained with cPCR and qPCR were 20.2% (36/178) and 38.8% (69/178), respectively, with 11.8% (21/178) testing positive in both molecular assays. Overall, 87.1% (155/178) were positive for anti-Leishmania spp. antibodies and/or Leishmania spp. DNA. Positivity to one or more tests was statistically associated with lymphadenomegaly, skin lesions, lymphocytosis, anemia and hyperproteinemia. The results of this study revealed a high level of exposure to Leishmania in stray dogs in an area of intense human visceral leishmaniasis transmission, suggesting that dogs play a role as reservoirs in the transmission cycle of this zoonosis.
Subject
General Veterinary,Parasitology
Reference25 articles.
1. Leishmaniasis worldwide and global estimates of its incidence;Alvar J;PLoS One,2012
2. Quality of diagnosis of canine visceral leishmaniasis in epidemiological surveys: an epidemic in Belo Horizonte, Minas Gerais, Brazil, 1993-1997;Alves WA;Cad Saude Publica,2004
3. Laboratory procedures for veterinary technicians.;Anthony E,2007
4. Epidemiological aspects and risk factors for infection by Leishmania infantum chagasi in dogs from municipality of Petrolina, Northeastern Brazil;Araujo AC;Vet Parasitol Reg Stud Reports,2016
5. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection;Arruda MM;Mem Inst Oswaldo Cruz,2016
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献