Affiliation:
1. Universidad Nacional de Asunción, Paraguay
Abstract
Abstract To verify the occurrence of natural Trypanosoma cruzi infection in non-human primates from a rural endemic area of the east region of Paraguay, xenodiagnosis was performed in 35 animals belonging to two species. For genotyping and T. cruzi discrete typing unit (DTU) assignment, a combination of four markers was used, including amplification products of the small (18S) and large (24Sα) subunits of ribosomal ribonucleic acid gene, the intergenic region of mini-exon gene and the heat shock protein 60 Eco-RV polymerase chain reaction-restriction fragment length polymorphism (HSP60/EcoRV-PCR-RFLP). One specimen of Sapajus cay was found positive and infected by the DTU TcII. This result constitutes the first record of natural T. cruzi infection in a sylvatic monkey in Paraguay, harbouring a DTU associated with severe Chagas disease in humans.
Subject
General Veterinary,Parasitology
Reference42 articles.
1. Characterization of Paraguayan Trypanosoma cruzi strains isolated from acute patients of Chagas disease;Acosta N;Trop Med Parasitol,1995
2. Isoenzyme profiles of Trypanosoma cruzi stocks from different areas of Paraguay;Acosta N;Mem Inst Oswaldo Cruz,2001
3. Explosive Pleistocene range expansion leads to widespread Amazonian sympatry between robust and gracile capuchin monkey;Alfaro JW;J Biogeogr,2011
4. How different are robust and gracile capuchin monkeys? An argument for the use of Sapajus and Cebus.;Alfaro JWL;Am J Primatol,2012
5. The experimental infection of Cebus apella sp. monkeys with Trypanosoma cruzi. Its clinical, electrocardiographic and anatomicopathological assessment;Almeida EA;Rev Soc Bras Med Trop,1992
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献