PYROLIGNEOUS LIQUOR EFFECT ON IN AND EX VITRO PRODUTION OF Oeceoclades maculata (Lindl). Lindl.1

Abstract

ABSTRACT Pyroligneous fraction from wood carbonization can be used for several purposes. In this sense, this study aimed to test a pyroligneous liquor extracted during carbonization of forest residues for the vegetative development of the orchid Oeceoclades maculata. The concentrations of pyroligneous liquor used in the treatments were T1 = 0 mL L−1, T2 = 1 mL L−1, T3 = 2 mL L−1, T4 = 3 mL L−1, T5 = 4 mL L−1, and T6 = 5 mL L−1. These treatments were tested together with and without activated charcoal for in vitro and ex vitro propagation. In vitro developed protocorms were assessed according to development or not, the presence of fungus, leaves, and roots. For ex vitro evaluations, leaf size, size, the number of roots, total plant height, and the number of buds were assessed. Possible differences between treatments were tested by means of analysis of variance (ANOVA) and Tukey’s test at 5% significance. The use of pyroligneous liquor at low doses was significant in vegetative tissue development as well as for root and leaf formations in O. maculata seedlings since it presents all the essential mineral elements to the structural development of these plants.