Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies-Reference-Cited by-同舟云学术

Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies

Published:2010-10 Issue:10 Volume:30 Page:877-880
ISSN:0100-736X
Container-title:Pesquisa Veterinária Brasileira
language:
Short-container-title:Pesq. Vet. Bras.

Author:

Mazur Carlos¹,Reis Jenner K.P²,Leite Romulo C²,Danelli Maria das Graças M¹,Hagiwara Mitika K³,Góes Ana Carolina M.A. de⁴,Medeiros Marcos A⁴

Affiliation:

1. Universidade Federal Rural do Rio de Janeiro (UFRRJ), Brazil

2. Universidade Federal de Minas Gerais, Brazil

3. Universidade de São Paulo (USP), Brazil

4. Fundação Oswaldo Cruz, Brazil

Abstract

Feline Immunodeficiency Virus is a worldwide infection and is considered a significant pathogen. The diagnosis of FIV infections is mainly based on commercially available rapid tests that are highly expensive in Brazil, hence it is rarely performed in the country. Furthermore, lentiviruses grow slowly and poorly in tissue cultures, making the production of viral antigen by classic means and thus the establishment of FIV immunodiagnosis impracticable. In order to deal with this, recombinant DNA techniques were adopted to produce the protein p24, a viral capsid antigen. The protein's reactivity evaluation analyzed by Western blot indicated that this recombinant antigen can be a useful tool for the immunodiagnostic of FIV infections.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

Link

http://www.scielo.br/pdf/pvb/v30n10/a08v30n10.pdf

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