Variability and genetic diversity study in an advanced segregating population of rice with bacterial blight resistance genes introgressed

Abstract

ABSTRACT Bacterial blight disease of rice caused by Xanthomonas oryzae pv. oryzae is one of the most important widespread disease around the globe and can cause severe yield reductions of up to 30% of the total yield. Genetic variability was studied in 54 families of CB 174 R × IRBB 60 rice with bacterial blight resistance genes introgressed at the Department of Rice, Tamil Nadu Agricultural University, Coimbatore, India. Traits such as plant height and the number of productive tillers per plant were found to have negative skewness in this study, indicating the duplication of gene effects. Panicle length and the number of grains per panicle were found to have positive kurtosis, indicating gene interactions. The phenotypic coefficient of variation was slightly higher than the genotypic coefficient of variation for all traits, indicating that the observed variation was not only due to genotypic but also due to the environmental effect on the expression of these traits. High heritability coupled with high genetic advance as a percent of the mean was observed for plant height. Moreover, moderate heritability coupled with high genetic advance as a percent of the mean was observed for the single plant yield of rice. Cluster and principal component analyses revealed that the 54 F2:3 families were grouped into three clusters based on six agro-morphological traits. Clusters III and II had higher mean values for most of the studied traits than cluster I. Intercrossing lines from distinct clusters can be identified and crossed to generate larger variability to produce desirable recombinants for higher yield with a wide genetic base.