Culture media and bap concentrations in the embryo culture of ‘BRS Kampai’ peach

Author:

Grigolo Chaiane Renata1ORCID,Citadin Idemir1ORCID,Oliveira Marisa De Cacia1ORCID,Scariotto Silvia1ORCID,Pertille Rafael Henrique1ORCID,Santos Ester Provensi1ORCID

Affiliation:

1. Universidade Tecnológica Federal do Paraná, Brasil

Abstract

Abstract Early-ripening peach cultivars present difficulties in the process of natural germination of seeds. In order to solve this problem, the culture of embryos in vitro can be considered an alternative, in which seeds find conditions to complete germination and development in a satisfactory way. Different protocols and culture media have been tested to meet the nutritional needs of the embryo, but they still have problems and need to be optimized. The objective of this work was to test culture media and BAP concentrations in order to increase the germination percentage of embryos and viable ‘BRS Kampai’ peach seedlings, avoiding possible anomalies in the process. The experiment was carried out at the Laboratory of Tissue Culture – “Universidade Tecnológica Federal do Paraná”, Campus of Pato Branco. The experimental design used was randomized blocks, in 2x5 factorial, with four replicates, with plot being represented by 15 embryos. The culture media tested were MS and WPM with five BAP concentrations (0, 1, 2, 3 and 4 mg L-1). In the in vitro period, seed germination was evaluated through the attribution of scores, and embryo development, measuring the length of stem and main root. During the acclimatization period, seedlings were evaluated for survival, stem length, viable seedlings and rosette formation. Considering the conditions under which the experiment was conducted, it was concluded that MS culture medium with the addition of 1 mg L-1 of BAP allowed higher germination percentage and viable ‘BRS Kampai’ peach seedlings.

Publisher

FapUNIFESP (SciELO)

Subject

Plant Science,Agronomy and Crop Science,Food Science

Reference33 articles.

1. Resposta do sistema antioxidativo sob a influência de 6-benzilaminopurina na micropropagação de cana-de-açúcar.;ANDRADE G.R.,2011

2. Relationships of fruit development period, seed germination, seedling survival and percent dry weight of ovule in peach.;BACON T.A.;HortScience,1995

3. Eliminação de anomalias fisiológicas, in vitro, de plântulas de pessegueiro.;BARBOSA W.;Bragantia,1989

4. Cultura de embriões in vitro para o melhoramento de pessegueiros precoces.;BARBOSA W.;Bragantia,1985

5. Vegetative propagation of Alberta magna by tissue culture and grafting.;BEN-JAACOV J.;HortScience,1991

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding;Plant Cell, Tissue and Organ Culture (PCTOC);2023-12-14

2. Embryo rescue in stone fruit breeding: specifics and application success;Horticulture and viticulture;2023-11-30

3. The heat distribution of the underdamped Langevin equation;Physica A: Statistical Mechanics and its Applications;2023-04

4. Heat fluctuations in overdamped non-isothermal processes;The European Physical Journal B;2023-02

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3