FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)

Author:

AGUILERA PATRICIA M.1,DEBAT HUMBERTO J.2,SCALDAFERRO MARISEL A.3,MARTÍ DARDO A.1,GRABIELE MAURO1

Affiliation:

1. Universidad Nacional de Misiones, Argentina

2. Instituto Nacional de Tecnología Agropecuaria, Argentina

3. Universidad Nacional de Córdoba, Argentina

Abstract

ABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus.

Publisher

FapUNIFESP (SciELO)

Subject

Multidisciplinary

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