Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria-Reference-Cited by-同舟云学术

Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria

Published:2016-09 Issue:3 Volume:14 Page:366-373
ISSN:1679-4508
Container-title:Einstein (São Paulo)
language:
Short-container-title:Einstein (São Paulo)

Author:

Correia Rodolfo Patussi¹,Bento Laiz Cameirão¹,Bortolucci Ana Carolina Apelle¹,Alexandre Anderson Marega¹,Vaz Andressa da Costa¹,Schimidell Daniela¹,Pedro Eduardo de Carvalho¹,Perin Fabricio Simões¹,Nozawa Sonia Tsukasa¹,Mendes Cláudio Ernesto Albers¹,Barroso Rodrigo de Souza¹,Bacal Nydia Strachman²

Affiliation:

1. Hospital Israelita Albert Einstein, Brazil

2. Hospital Israelita Albert Einstein, Brazil; Centro de Hematologia de São Paulo, Brazil

Abstract

ABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.

Publisher

FapUNIFESP (SciELO)

Subject

General Medicine

Link

http://www.scielo.br/pdf/eins/v14n3/1679-4508-eins-14-03-0366.pdf

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