Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae and Streptococcus sp. by polymerase chain reaction for the diagnosis of bacterial meningits

Author:

Failace Luciane1,Wagner Mario2,Chesky Marisa1,Scalco Rosana1,Jobim Luiz Fernando3

Affiliation:

1. Universidade Federal do Rio Grande do Sul

2. UFRS; London University

3. UFRS; Hospital de Clínicas de Porto Alegre; Laboratório DNA Reference, Brazil

Abstract

The simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus sp. was assessed by polymerase chain reaction (PCR) for the diagnosis of bacterial meningitis, as well as the applicability of PCR as a routine test. A cohort study was carried out with 182 children (2 months to 12 years of age) with suspicion of bacterial meningitis. Routine tests identified the etiologic agent in 65/84 children whose clinical status and laboratory findings suggested the presence of bacterial meningitis. Bacterial meningitis was ruled out in 98 children. In 19 children, the etiologic diagnosis was not possible using standard methods; in 14 of these patients, the etiologic agent was identified by PCR (N. meningitidis=12; H. influenzae=1; Streptococcus sp.=1). The sensitivity of PCR was 88.1%; specificity, 99.0%; positive predictive value, 98.7%; and negative predictive, 90.1%. PCR is a useful complementary diagnostic technique, especially when Gram stain, culture, or antigenic detection are negative or inconclusive.

Publisher

FapUNIFESP (SciELO)

Subject

Neurology,Clinical Neurology

Reference13 articles.

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2. Bacterial meningitis in the United States, 1978 through 1981: the national bacterial meningitis surveillance study;Schelch WF;JAMA,1985

3. Long-term sequelae of childhood acute bacterial meningitis in a developing country: a study from the Sudan;Salih MA;Scand J Infect Dis,1991

4. Control of meningococcal infection in the African meningitis belt by selective vaccination;Greenwood BM;Lancet,1980

5. Laboratory diagnosis of bacterial meningitis;Gray LD;Clin Microbiol Rev,1992

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