Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers-Reference-Cited by-同舟云学术

Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

Published:2010-09 Issue:3 Volume:10 Page:204-210
ISSN:1984-7033
Container-title:Crop Breeding and Applied Biotechnology
language:
Short-container-title:Crop Breed. Appl. Biotechnol.

Author:

Rocha Elizângela Almeida¹,Paiva Luciano Vilela²,Carvalho Humberto Henrique de³,Guimarães Claudia Teixeira⁴

Affiliation:

1. Universidade Federal de Minas Gerais, Brazil

2. Universidade Federal de Lavras, Brazil

3. Universidade Federal de Viçosa, Brazil

4. Embrapa Milho e Sorgo, Brazil

Abstract

This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.

Publisher

FapUNIFESP (SciELO)

Subject

General Medicine

Link

http://www.scielo.br/pdf/cbab/v10n3/a04v10n3.pdf

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