Endotoxins in surgical instruments of hip arthroplasty

Author:

Goveia Vania Regina1,Mendoza Isabel Yovana Quispe1,Guimarães Gilberto Lima1,Ercole Flavia Falci1,Couto Bráulio Roberto Gonçalves Marinho2,Leite Edna Marilea Meireles1,Stoianoff Maria Aparecida Resende1,Ferreira José Antonio Guimarães1

Affiliation:

1. Universidade Federal de Minas Gerais, Brazil

2. Centro Universitário de Belo Horizonte, Brazil

Abstract

Abstract OBJECTIVE To investigate endotoxins in sterilized surgical instruments used in hip arthroplasties. METHOD A descriptive exploratory study conducted in a public teaching hospital. Six types of surgical instruments were selected, namely: acetabulum rasp, femoral rasp, femoral head remover, chisel box, flexible bone reamer and femoral head test. The selection was based on the analysis of the difficulty in removing bone and blood residues during cleaning. The sample was made up of 60 surgical instruments, which were tested for endotoxins in three different stages. The EndosafeTM Gel-Clot LAL (Limulus Amebocyte Lysate method) was used. RESULT There was consistent gel formation with positive analysis in eight instruments, corresponding to 13.3%, being four femoral rasps and four bone reamers. CONCLUSION Endotoxins in quantity ≥0.125 UE/mL were detected in 13.3% of the instruments tested.

Publisher

FapUNIFESP (SciELO)

Subject

General Nursing

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