Restriction enzyme analysis of the chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro

Author:

Echeverrigaray Sergio1,Carvalho Maria Tereza Vitral2,Derbyshire Eric

Affiliation:

1. Universidade de Caxias do Sul

2. Universidade de São Paulo

Abstract

The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to identified more than 20 fragments of 18 to 0.65kb. The size of Phaseolus vulgaris L. cp DNA was estimated in 140kb with the presence of a repeat sequence of about 22kb.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Agronomy and Crop Science,Animal Science and Zoology

Reference7 articles.

1. The structure of chloroplast DNA;BEDBROOK J.R.;Ann Rev Plant Physiol,1979

2. Analysis of chloroplast genomes in small amounts of tissue;CLARK E.M.;Plant Molec Biol Rep,1983

3. Plant molecular systematics: molecular approaches;CRAWFORD D.J.,1990

4. Themolecular size and conformation of the chloroplast DNA from higher plants;KOLODNER R.;Biochim Biophys Acta,1975

5. A miniprep system for analysis of chloroplast DNA restriction enzyme digest fragments in Nicotina;KUT S.A.;Plant Molec Biol Rep,1986

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