Rapid serum agglutination and agar gel immunodiffusion tests associated to clinical signs in rams experimentally infected with Brucella ovis

Author:

Nozaki Cristiane Nakada1,Lira Nair Silva Cavalcanti de1,Augusto Filho Otávio1,Azevedo Hymerson Costa2,Rodello Leandro1,Dasso Maurício Gaudério3,Bicudo Sony Dimas1,Antunes João Marcelo Azevedo de Paula1,Megid Jane1

Affiliation:

1. Universidade Estadual Paulista, Brasil

2. Empresa Brasileira de Pesquisa Agropecuária, Brasil

3. Fundação Estadual de Pesquisa Agropecuária, Brasil

Abstract

The purpose of this study was to evaluate the agar gel immunodiffusion and the rapid serum agglutination tests associated to clinical signs in rams experimentally infected with Brucella ovis. The serological profile during the 12 months of infection showed a large fluctuation of antibodies that favors the failure in the diagnostic. The evaluation of tests after the experimental infection allowed to suggest that none of the tests were able to detect the infection throughout the period of study. The study reinforces the importance of considering the clinical signs to support the diagnosis of Brucella ovis infection in rams.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Agronomy and Crop Science,Animal Science and Zoology

Reference24 articles.

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2. Tratado de patologia y producción ovina;BLASCO M.J.M.,1990

3. Brucella ovis excretion in semen of serum negative, clinically normal breeding rams;BULGIN M.S.;Journal of the American Veterinary Medical Association,1990

4. Recent advances in the study of brucella antigens and their serological cross-reactions;CORBEL M.J.;Veterinary Bulletin,1985

5. Bacterial isolates associated with epididymitis in rams from Idaho and Eastern Oregon flocks;DELONG W.J.;American Journal of Veterinary Research,1979

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