Histological and ultrastructural studies on the effect of Cassia alata methanolic leaf extracts against chemically induced lung adenocarcinoma in rats

Author:

Fathalla A.S.1ORCID,Ibrahim M.A.1ORCID,Mohamed S.R.1ORCID,Dkhil M.A.2ORCID,Thagfan F.A.3ORCID,Abdel-Gaber R.4ORCID,Soliman D.1ORCID

Affiliation:

1. Helwan University, Egypt

2. Helwan University, Egypt; Applied Science Private University, Jordan

3. Princess Nourah bint Abdulrahman University, Saudi Arabia

4. King Saud University, Saudi Arabia

Abstract

ABSTRACT The present work aims to evaluate anticancer performance of Cassia alata methanolic leaf extracts (CMLE) in ethyl carbamate-stimulated lung adenocarcinoma (LAD) in differentiation to the function of Cisplatin (CIPL). Rats were divided into four groups: (1) control (CONT), (2) lung-adenocarcinoma (LAD) injected intra-peritoneally with 1g/kg ethyl carbamate once weekly for a month, (3) LAD+CMLE administered 500 mg/kg CMLE orally for the last two months of the experiment, and (4) LAD+CIPL treated group, injected 2.5 mg/kg Cisplatin intraperitoneally once weekly for the last two months of the experiment. Light and electron microscopic examinations revealed adenocarcinoma development in terminal bronchiole besides some histopathological changes in the LAD group such as atypical, exaggerated collagen fibers, increment of mucinous content, and increasing of PCNA positive immunoreactivity whereas electron microscopy investigation exposed that papillary adenocarcinoma originated from Clara cells in the LAD group. The LAD+CMLE treated group showed no tumor masses and nearly all with normal lung histology. It also recovered the normal ultrastructure of bronchiolar Clara cells. CMLE treatment offers a new alternative cure with less toxicity than Cisplatin for lung cancer therapy. Hence, CMLE would be employed as a novel supply of anti-cancer compounds combating lung cancer.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

Reference56 articles.

1. Phytochemistry and antibacterial activity of Senna alata flower;ADEDAYO O.;Pharm. Biol.,2001

2. Ameliorative effects of silymarin on HCl-induced acute lung injury in rats, role of the Nrf-2/HO-1 pathway;AHMED R.F.;Iran J. Basic Med. Sci.,2019

3. Phytochemical, cytotoxicity and antioxidant investigation of Cassia alata leaves growing in Egypt;ALI M.I.;J. Innov. Pharm. Biol. Sci.,2017

4. Carnitine deficiency provokes cisplatin-induced hepatotoxicity in rats;AL-MAJED A.A;Basic Clin. Pharmacol. Toxicol.,2007

5. The anticancer role of capsaicin in experimentally induced lung carcinogenesis;ANANDAKUMAR P.;J. Pharmacopuntura,2015

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3