Caprine lentivirus in sheep milk and semen-Reference-Cited by-同舟云学术

Caprine lentivirus in sheep milk and semen

Published:2017-04 Issue:2 Volume:69 Page:391-397
ISSN:0102-0935
Container-title:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
language:
Short-container-title:Arq. Bras. Med. Vet. Zootec.

Author:

Lima C.C.V.¹,Ayres M.C.C.¹,Pinheiro R.R.²,Costa J.N.³,Andrioli A.²,Souza T.S.³,Azevedo D.A.A.⁴,Santos V.W.S.⁵,Araújo J.F.⁶,Sousa A.L.M.⁶,Peixoto R.M.⁴,Damasceno E.M.⁶,Costa Neto A.O.⁷

Affiliation:

1. Universidade Federal da Bahia, Brazil

2. Empresa Brasileira de Pesquisa Agropecuária, Brazil

3. Universidade Federal do Recôncavo da Bahia, Brazil

4. Universidade Estadual do Ceará, Brazil

5. Universidade Federal Rural do Semiárido, Brazil

6. Universidade Estadual do Vale do Acaraú, Brazil

7. Universidade Estadual de Santana, Brazil

Abstract

ABSTRACT With the objective of detecting the presence of caprine lentivirus (CLV) in ewe milk and in ram semen, ten matrixes and four reproducers experimentally infected with CLV were used. Samples of ewe milk were collected during the four months of lactation, five collections per animal, totaling 50 samples. Regarding the rams, eight semen collections were made per animal, during one year of experimentation, totaling 32 samples. The milk and semen samples were submitted to DNA extraction and the nested polymerase chain reaction test (nPCR) to detect CLV proviral DNA. Eight (16%) of the milk samples were positive in nPCR originating from two ewes. Only one (3.12%) semen sample was positive. The amplification products were sequenced, and were confirmed to be a CLV genomic sequence. Thus, the presence of CLV proviral DNA in sheep milk and semen was demonstrated, confirming the feasibility of infection between species, and alerting to the risk of spreading infections.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

Link

http://www.scielo.br/pdf/abmvz/v69n2/0102-0935-abmvz-69-02-00391.pdf

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