Production and characterization of galactose oxidase produced by four isolates of Fusarium graminearum

Author:

Gasparotto Elainy Patrícia Lino1,Abrão Sorrailla Carolina Carrard1,Inagaki Suely Yaeko1,Tessmann Dauri José2,Kemmelmeier Carlos1,Tessmann Ione Parra Barbosa1

Affiliation:

1. Universidade Estadual de Maringá

2. Universidade Estadual de Maringá, Brasil

Abstract

A screening aimed to find new galactose oxidase producer isolates and to evaluate the production among Fusarium graminearum strains was conducted. Thirty-five isolates out of 39 analysed produced the enzyme at several levels. The data indicated a wide distribution of galactose oxidase within F. graminearum and also revealed new producer isolates. The enzyme produced by different isolates showed similar thermal activity and stability and were active on same substrates. However, the optimum pH ranged from 7.0 to 7.5. Thus, all evaluated isolates were suitable for the production of galactose oxidase.

Publisher

FapUNIFESP (SciELO)

Subject

Multidisciplinary

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