Morphological alterations and G0/G1 cell cycle arrest induced by curcumin in human SK-MEL-37 melanoma cells-Reference-Cited by-同舟云学术

Morphological alterations and G0/G1 cell cycle arrest induced by curcumin in human SK-MEL-37 melanoma cells

Published:2010-04 Issue:2 Volume:53 Page:343-352
ISSN:1516-8913
Container-title:Brazilian Archives of Biology and Technology
language:
Short-container-title:Braz. arch. biol. technol.

Author:

Carneiro Marcella Lemos Brettas¹,Porfírio Elaine Paulucio²,Otake Andréia Hanada³,Chammas Roger³,Báo Sônia Nair²,Guillo Lidia Andreu¹

Affiliation:

1. Universidade Federal de Goiás, Brasil

2. Universidade de Brasília, Brasil

3. Universidade de São Paulo, Brasil

Abstract

The aim of this work was to study the effect of curcumin on cell cycle in the human SK-MEL-37 melanoma cell line. In addition, morphological and structural analyses were also performed. Flow cytometric analysis showed a G0/G1 arrest at 5 µM after 24 h exposure and a concentration-dependent increase in the proportion of sub-G0 hypodiploid cells. Typical apoptotic events were also observed by the fluorescence microscopy, transmission and scanning electronic microscopy. Loss of mitochondrial membrane potential was not detected. Results suggested that curcumin could arrest human melanoma cells at G0/G1 phase and induce a mitochondrial-independent apoptotic pathway.

Publisher

FapUNIFESP (SciELO)

Subject

Multidisciplinary

Link

http://www.scielo.br/pdf/babt/v53n2/13.pdf

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