In vitro establishment of blackberry (Rubus sp.) cultivar 'Xavante'

Author:

Pelizza Tânia Regina1,Silveira Fabiane Nunes2,Ribeiro Roberta Sabatino3,Machado Bruno Dalazen2,Rufatto Leo4,Kretzschmar Aike Anneliese4

Affiliation:

1. Universidade Federal da Fronteira Sul (UFFS, Brazil

2. Instituto Federal de Santa Catarina (IFSC, Brazil

3. Emater/RS-ASCAR, Brasil

4. Universidade do Estado de Santa Catarina (UDESC, Brazil

Abstract

ABSTRACT: The objective of this study was to evaluate the in vitro establishment of 'Xavante' blackberry under different conditions of plant luminosity and concentrations of salts of MS medium. The maintenance of 'Xavante' blackberry plants in the absence of light, with the use of MS medium with salt concentrations of 75% and 100% reduce bacterial contamination and the percentage of explants oxidized and provide the highest percentage of surviving and established explants. The 50% salt dilution in MS medium is not recommended when plants are subjected to the absence of light due to the high percentage of oxidized explants and consequently lower survival and establishment.

Publisher

FapUNIFESP (SciELO)

Reference9 articles.

1. In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume.;ASSIS K.C. de;Acta Scientiarum Agronomy,2012

2. Etiolation to clonal plant propagation.;BIASI L.A.;Ciência Rural,1996

3. Micropropagation of '420-A' grapevine rootstock.;DZAZIO P.M.;Revista Brasileira de Fruticultura,2002

4. Micropropagação;GRATTAPAGLIA D.,1998

5. A revised médium for rapid growth and bioassays with tobacco tissue cultures.;MURASHIGE T.;Physiologia plantarum,1962

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